Three‐dimensional multiphoton/optical coherence tomography for diagnostic applications in dermatology |
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Authors: | Aneesh Alex Jessika Weingast Martin Weinigel Marcel Kellner‐Höfer Romina Nemecek Michael Binder Hubert Pehamberger Karsten König Wolfgang Drexler |
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Institution: | 1. Centre for Medical Physics and Biomedical Engineering, Medical University Vienna, Austria;2. Division of General Dermatology, Department of Dermatology, Medical University Vienna, Austria;3. JenLab GmbH, Schillerstra?e 1, Jena, Germany;4. Department of Biophotonics and Technology, Saarland University, Saarbrücken, Germany |
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Abstract: | A preliminary clinical trial using state‐of‐the‐art multiphoton tomography (MPT) and optical coherence tomography (OCT) for three‐dimensional (3D) multimodal in vivo imaging of normal skin, nevi, scars and pathologic skin lesions has been conducted. MPT enabled visualization of sub‐cellular details with axial and transverse resolutions of <2 μm and <0.5 μm, respectively, from a volume of 0.35 × 0.35 × 0.2 mm3 at a frame rate of 0.14 Hz (512 × 512 pixels). State‐of‐the‐art OCT, operating at a center wavelength of 1300 nm, was capable of acquiring 3D images depicting the layered architecture of skin with axial and transverse resolutions ~8 μm and ~20 μm, respectively, from a volume of 7 × 3.5 × 1.5 mm3 at a frame rate of 46 Hz (1024 × 1024 pixels). This study demonstrates the clinical diagnostic potential of MPT/OCT for pre‐screening relatively large areas of skin using 3D OCT to identify suspicious regions at microscopic level and subsequently using high resolution MPT to obtain zoomed in, sub‐cellular level information of the respective regions (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
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Keywords: | Multiphoton tomography optical coherence tomography dermatology three dimensional non‐invasive in vivo imaging multimodal imaging |
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