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海洋环境中难培养微生物的寡营养培养
引用本文:田 甜,李冬梅,戴世鲲,殷克东,孙慧敏,李 翔.海洋环境中难培养微生物的寡营养培养[J].微生物学通报,2009,36(7):1031-1039.
作者姓名:田 甜  李冬梅  戴世鲲  殷克东  孙慧敏  李 翔
作者单位:1. 中科院南海海洋研究所中科院海洋生物可持续利用重点实验室,广东省海洋药物重点实验室,广东,广州,510301;中国科学院南海海洋研究所,热带海洋环境动力学实验室,广东,广州,510301;中国科学院研究生院,北京,100049
2. 中国科学院南海海洋研究所,热带海洋环境动力学实验室,广东,广州,510301;中国科学院研究生院,北京,100049
3. 中科院南海海洋研究所中科院海洋生物可持续利用重点实验室,广东省海洋药物重点实验室,广东,广州,510301;中国科学院研究生院,北京,100049
4. 中国科学院南海海洋研究所,热带海洋环境动力学实验室,广东,广州,510301
5. 中科院南海海洋研究所中科院海洋生物可持续利用重点实验室,广东省海洋药物重点实验室,广东,广州,510301
基金项目:国家基金委基金项目(No. 40676074); 大洋协会项目(No. DYXM115-02)
摘    要:海洋中存在着丰富的微生物资源, 但迄今为止能够在实验室培养的微生物却不到1%, 而且能够通过培养得到的环境优势种更少, 这成为当代环境微生物学研究和海洋资源开发的最大障碍。过去十多年来, 通过不断改进培养方法和检测手段, 发明了许多新颖独特的技术, 提高了培养效率。特别是通过海洋微生物的寡营养培养技术, 分离并命名了一些难培养微生物, 给予人们极大的启发。海洋微生物资源的可持续性开发和利用, 是21世纪人类发展的重要方向, 是我们研究海洋微观世界的基础, 值得微生物学界同仁的共同关注。

关 键 词:难培养细菌    寡营养培养    海洋微生物    系统分类学

Culture Methods of the Oligotrophic Marine Microbes
TIAN Tian,LI Dong-Mei,DAI Shi-Kun,YIN Ke-Dong,SUN Hui-Min and LI Xiang.Culture Methods of the Oligotrophic Marine Microbes[J].Microbiology,2009,36(7):1031-1039.
Authors:TIAN Tian  LI Dong-Mei  DAI Shi-Kun  YIN Ke-Dong  SUN Hui-Min and LI Xiang
Institution:(1. Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Provincial Key Laboratory of Marine Materia Medica, Guangzhou, Guangdong 510301, China) (2. Key Laboratory of Tropical Marine Environmental Dynamics, South China Sea Institute o;(2. Key Laboratory of Tropical Marine Environmental Dynamics, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, Guangdong 510301, China) (3. Graduate University of the Chinese Academy of Sciences, Beijing 100049, China);(1. Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Provincial Key Laboratory of Marine Materia Medica, Guangzhou, Guangdong 510301, China) (3. Graduate University of the Chinese Academy of Sciences, Beijing 100049, China);Key Laboratory of Tropical Marine Environmental Dynamics, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, Guangdong 510301, China;(1. Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Provincial Key Laboratory of Marine Materia Medica, Guangzhou, Guangdong 510301, China) (3. Graduate University of the Chinese Academy of Sciences, Beijing 100049, China);Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Provincial Key Laboratory of Marine Materia Medica, Guangzhou, Guangdong 510301, China
Abstract:Molecular methods and fluoroscopic techniques suggest that rich microbial diversity exist in the marine environment, but less than 1% of these microbes can be cultured in the laboratory conditions, and that the cultivable dominant species were even less. This limitation has long been a barrier to the development of environmental microbiology and the utilization of marine resources. In the past decade, novel methods for culture and detection of these uncultured marine microbes have successfully applied to obtain several conventionally-uncultured microbes including those from extreme environments. Those progresses have inspired researchers greatly. Developments in the research of marine microbial resources are an important basis for the study of the micro-world and deserve increasing scientific attention.
Keywords:Uncultured microorganisms  Oligo-culture  Marine microbes  Phylogenetics
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