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In vitro studies on Eucalyptus globulus rooting ability
Authors:H Trindade  M S Pais
Institution:(1) Soporcel Forestry Research Centre, P.O. Box 15, 2065 Alcoentre, Portugal;(2) Center for Plant Biotechnology, Department of Plant Biology, Faculty of Sciences, University of Lisboa, Bloco C2, Campo Grande, 1700 Lisboa, Portugal
Abstract:Summary Micropropagation has the potential to quickly introduce selected genotypes of adult Eucalyptus globulus clones and it is now widely used in Portugal as a part of genetic improvement programs. Several clones have been established and multiplied in vitro. The different clones have individual requirements for successful rooting. Rejuvenation was achieved at different periods after culture initiation for the different clones. Subculturing preceding rooting in multiplication medium supplemented with riboflavin and cholene chloride allowed the increase of rooting ability for several clones tested. Removal of boron from the rooting medium increased rooting by 10%. Indolebutyric acid (IBA) dipping before transfer to the rooting medium resulted in a rooting percentage of 80–95% for the best clones tested. Acclimatization was performed without difficulties (90–95% success) and the rooted plants were either planted directly or used as mother plants for further cutting production, depending on the needs. The results described in this paper increase the commercial feasibility of the micropropagation system for E. globulus.
Keywords:rooting  boron  rejuvenation            Eucalyptus globulus
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