| 分选酶的研究进展及其在生物技术中的应用 |
| |
| 作者姓名： | 姜利滨 文莹 陈芝 |
| |
| 作者单位： | 中国农业大学农业生物技术国家重点实验室, 北京 100193 |
| |
| 基金项目： | 农业部微生物资源与利用开放重点实验室青年基金项目(201002)资助。 |
| |
| 摘 要： | 分选酶(sortase)普遍存在于革兰氏阳性细菌中,是一类膜结合的转肽酶,负责将表面蛋白共价结合到细胞壁的肽聚糖上。由于其独特的作用机制,分选酶在生物技术领域具有广阔的应用前景,可应用于革兰氏阳性菌的表面展示、蛋白质工程等。
|
| 关 键 词： | 分选酶 表面蛋白 表面展示 蛋白质连接 |
| 收稿时间： | 2011-05-11 |
Research Progress on Sortase and its Application in Biotechnology |
| |
| Authors: | JIANG Li-bin WEN Ying CHEN Zhi |
| |
| Institution: | State Key Laboratories for Agro-Biotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, China |
| |
| Abstract: | Sortases are membrane-bound transpeptidases produced by Gram-positive bacteria to anchor cell surface proteins covalently to peptidoglycan in the cell wall. Due to its specific reaction, sortases are exploited for a range of biotechnology application, such as Gram-positive bacterial cell-surface display and protein engineering, etc. This paper reviews research progress on sortase and its application in biotechnology. |
| |
| Keywords: | sortase surface protein surface display protein linkage |
|
| 点击此处可从《》浏览原始摘要信息 |
| 点击此处可从《》下载免费的PDF全文 |
