A novel <Emphasis Type="Italic">Candida glycerinogenes</Emphasis> mutant with high glycerol productivity in high phosphate concentration medium |
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Authors: | Bin?Zhuge Xue-Na?Guo Crispen?Mawadza Hui-Ying?Fang Xue-Ming?Tang Xi-Hong?Zhang Email author" target="_blank">Jiang?ZhugeEmail author |
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Institution: | (1) School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Huihe Road No.170, Wuxi, Jiangsu Province, 214036, P. R. China;(2) Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland, 7602, South Africa |
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Abstract: | A novel Candida glycerinogenes mutant, which possesses high glycerol productivity in a high phosphate concentration medium, was obtained by mutagenesis of an industrial glycerol producer. The mutant accumulated a total biomass of 11.5 g l−1, which is less than the 15 g l−1of the wild-type strain, but it consumed glucose faster than the wild-type strain did. The mutant reached its maximal glycerol concentration of 129 g l−1 in 84 h compared to 96 h for the wild-type strain. High cytoplasmic glycerol-3-phosphate dehydrogenase activity of the mutant in the early glycerol formation phase, leading to a rapid glycerol synthesis and accumulation, may be the main reason for the short fermentation process. |
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Keywords: | Candida glycerinogenes mutant cytoplasmic glycerol-3-phosphate dehydrogenase (ctGPD) glycerol phosphate |
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