Semi-conservative synthesis of DNA in UV-sensitive mutant cells of Chinese hamster after UV-irradiation |
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| Authors: | F L Vikhanskaya I A Khrebtukova E S Manuilova |
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| Institution: | 1. Laboratory of Radiation Cytology, Institute of Cytology, Academy of Sciences of the U.S.S.R., 199121 Maklin Ave. 32, Leningrad, U.S.S.R.;2. Laboratory of Somatic Cell Genetics, Institute of Molecular Genetics, Academy of Sciences of the U.S.S.R., 123182, Kurchatov Sq. 46, Moscow U.S.S.R. |
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| Abstract: | A study was made of the rate of semi-conservative DNA synthesis in asynchronous UV-resistant (clone V79) and UV-sensitive clones (VII and XII) of Chinese hamster cells after UV-irradiation. In all 3 clones studied, UV-irradiation (5-30 J/m2) induced a decrease in the rate of DNA synthesis during the subsequent 1-2 h. In the resistant clone (V79) recovery of DNA synthesis rate started after the first 2 h post-irradiation (5 J/m2) and by the 3rd hour reached its maximum value, which constituted 70% of that observed in control, non-irradiated cells. The UV-sensitive mutant clones VII and XII showed no recovery in the rate of DNA synthesis during 6-7 h post-irradiation. The results obtained show that the survival of cells is correlated with the ability of DNA synthesis to recover after UV-irradiation in 3 clones studied. The observed recovery of UV-inhibited DNA synthesis in mutant clones may be due to certain defects in DNA repair. |
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