DNA strand scission by the antitumor protein neocarzinostatin |
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Authors: | T A Beerman I H Goldberg |
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Institution: | Department of Pharmacology Harvard Medical School Boston, Massachusetts 02115 USA |
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Abstract: | The antibiotic protein, neocarzinostatin, induces the scission of DNA strands and . HeLa cell DNA prelabelled with 14C] thymidine is cut into large pieces with a peak at 80–90S when cells are incubated with 0.5 to 5.0 μg/ml of highly purified neocarzinostatin. Incubation of the antibiotic (0.5 μg/ml) with 3H] SV40 DNA in the presence of 2-mercaptoethanol results in the conversion of superhelical DNA I to nicked circular duplex DNA II. At high levels of drug, smaller fragments of linear DNA are produced. Strand breaks are detected in both neutral and alkaline sucrose gradients, indicating that drug susceptibility is not due to alkali-labile bonds. |
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