Construction of a human cytochrome c gene and its functional expression in Saccharomyces cerevisiae |
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| Authors: | Y Tanaka T Ashikari Y Shibano T Amachi H Yoshizumi H Matsubara |
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| Institution: | Institute for Fundamental Research, Suntory, Ltd., Osaka. |
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| Abstract: | The nucleotide sequences of a partial cDNA and three pseudogenes of human cytochrome c were determined. The complete nucleotide sequences which encode human cytochrome c were constructed on the basis of one of the pseudogenes by in vitro mutagenesis. The constructed human cytochrome c was functionally expressed in Saccharomyces cerevisiae. The recombinant human cytochrome c was purified and characterized. |
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