Epidermal growth factor (EGF)-induced morphological changes in the basement membrane of chick embryonic skin |
| |
Authors: | Yoshihiro Akimoto Akiko Obinata Hiroyoshi Endo Professor Hiroshi Hirano |
| |
Affiliation: | (1) Department of Anatomy, Kyorin University School of Medicine, Tokyo, Japan;(2) Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan;(3) Department of Anatomy, Kyorin University School of Medicine, Mitaka, 181 Tokyo, Japan |
| |
Abstract: | Summary The effect of epidermal growth factor (EGF) on the basement membrane structure of chick embryonic skin cultured in a chemically defined medium (BGJb) containing 20 mM hydrocortisone, and EGF at 10, 50, or 100 ng/ml supplemented with 5% delipidized fetal calf serum, was examined by electron microscopy. During development of the epidermis in vitro, EGF (100 ng/ml) caused striking changes to occur in the basement membrane structure and in the keratinization process. The basement membrane frequently became discontinuous with many gaps apparent in section, and occasionally became folded following detachment from the basal surface of the epidermis and protruded into the underlying dermis. In the basal and intermediate cells of EGF-treated epidermis, tonofilament bundles were decreased in number, while desmosomes and hemidesmosomes revealed no significant changes in morphology. |
| |
Keywords: | Embryonic skin Epidermal growth factor Basement membrane Electron microscopy Chick |
本文献已被 SpringerLink 等数据库收录! |
|