Involvement of both the N-glycan-relevant and N-glycan-irrelevant structural elements in the recognition of human milk lactoferrin by the 1CF11 monoclonal antibody |
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Authors: | Fukaya Shinichi Shinoda Chisato Yabe Tomio Kanamaru Yoshihiro |
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Institution: | The United Graduate School of Agricultural Science, Gifu University, Gifu, Japan. |
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Abstract: | Monoclonal antibody 1CF11 has been suggested to specifically recognize a certain carbohydrate epitope shared by glycoproteins in human external secretions. We examined the effect of cleaving the polypeptide backbone and removing N-linked oligosaccharides on the reactivity with 1CF11 of human milk lactoferrin (hLf) to elucidate the structural features of the 1CF11 epitope. We reveal by treating hLF with trypsin and/or N-glycosidase that both the N-glycan-relevant and N-glycan-irrelevant structural elements were involved in the recognition of hLf by 1CF11. |
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