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Involvement of both the N-glycan-relevant and N-glycan-irrelevant structural elements in the recognition of human milk lactoferrin by the 1CF11 monoclonal antibody
Authors:Fukaya Shinichi  Shinoda Chisato  Yabe Tomio  Kanamaru Yoshihiro
Institution:The United Graduate School of Agricultural Science, Gifu University, Gifu, Japan.
Abstract:Monoclonal antibody 1CF11 has been suggested to specifically recognize a certain carbohydrate epitope shared by glycoproteins in human external secretions. We examined the effect of cleaving the polypeptide backbone and removing N-linked oligosaccharides on the reactivity with 1CF11 of human milk lactoferrin (hLf) to elucidate the structural features of the 1CF11 epitope. We reveal by treating hLF with trypsin and/or N-glycosidase that both the N-glycan-relevant and N-glycan-irrelevant structural elements were involved in the recognition of hLf by 1CF11.
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