Established cell lines from the beetleDiabrotica undecimpunctata (Coleoptera: Chrysomelidae) |
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Authors: | D E Lynn A Stoppleworth |
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Institution: | (1) USDA/ARS, Insect Pathology Lab, BARC-WEST, 20705 Beltsville, Maryland |
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Abstract: | Summary Two new cell lines, designated IPLB-DU182A and IPLB-DU182E, were developed from embryos of the southern corn rootworm,Diabrotica undecimpunctata. Cells were grown in the lepidopteran cell culture media IPL-52B and IPL-76 in a 3∶1 ratiowith 9% fetal bovine serum. The
IPL-52B was modified by deleting CaCl2·2H2O and NaHCO3 out of the formulation. The osmotic pressure was adjusted to the optimal osmolarity of 400 mOsm/kg by the addition of2 g mannitol/100 ml medium. The cells were primarily epithelial-like, but some spindle-shaped cells
were also present. The lines were 65% diploid and were characterized with respect to 10 isozymes. Cellscurrently grow with
a 5-d doubling time and are subcultured by trypsinization at 1-wk intervals and at a 1∶2 to 1∶5 split ratio. |
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Keywords: | southern corn rootworm Diabrotica undecimpunctata insect cell culture beetle |
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