山梨糖发酵产生2—酮基—L—古龙酸氮源代谢规律

对山梨糖发酵产生2-酮基-L-古龙酸氮源代谢规律进行了初步研究。通过对这一混合发酵体系蛋白和尿素代谢的研究表明,氮源代谢与单一菌体发酵相比有其特殊性,主要表现在尿素的加入有两个作用,即作为生理碱性物质调节体系pH和为菌体代谢提供部分氮源,而体系的蛋白含量随发酵时间的延续不断增加,其增加的原因是巨大芽孢杆菌由营养体转变成芽孢所致,这是该发酵体系的特点。本文还对该发酵体系各种氨基酸变化规律进行了讨论,将一共17种氨基酸按其变化规律分成了三类,较好地解释了各种氨基酸的变化情况,为进一步深入研究该体系的动力学特性提供了数据基础。

STUDIES ON METABOLISM OF NITROGEN SOURCE IN FERMENTATION OF 2-KETO-L-GULONIC ACID

The metabolism of nitrogen source was studied preliminarily in the fermentation of 2-keto-L-gulonic acid from sorbose. The study of this mixed population system is different from those of pure fermentation system. The addition of urea has two functions: to adjust pH as a physiological material and to provide nitrogen for the cell growth partially. The content of protein increases with fermentation time, the reason of this is due to the change of Bacillus megaterium from vegetative state into gemma, which is a feature of this system. The change of the concentrations of different amino acids is discussed, the changes of seventeen amino acids are divided into three categoris, which has been explained qualitatively. This study sets a base for further study of the kinetics of the system.