一株与同种13个型抗原广泛交叉的嗜肺军团菌

从太原市郊晋阳湖水分离到一株细菌,命名为Jin-1。其形态染色性、营养需要、生长特性、生化反应性、DNA和蛋白质分析结果均符合嗜肺军团菌鉴定要求。该株在IgM介导的两种凝集反应中与嗜肺军团菌14个血清群(型)标准株抗原普遍交叉,在IgG介导的IFA、ELISA、dot-ELISA中则呈现嗜肺军团菌血清群5特异性。鉴定Jin-1为抗原结构较标准株复杂的嗜肺军团菌5型。种、型鉴定结果已为CDC证实。在种内型间抗原交叉范围如此广泛的嗜肺军团菌尚未见于以往文献。因此认为Jin-1的交叉性抗原可能属于胸腺非依赖性抗原。

A LEGIONELLA PNEUMOPHILA STRAIN SEROLOGICALLY CROSS-REACTING WITH THIRTEEN SEROGROUPS OF THIS SPECIES

A bacterial strain, designated as Jin-1, was isolated from a water sample taken from the Jinyang Lake located in the suburbs of Taiyuan City, Shanxi Province. Its morphologic and tinctorial properties, nutritional requirements, growth characteristics, biochemical reactions, as well as results of DNA and peptide analysis overall met the criteria for identification of Legionella pneumophila. In serogrouping, extensive cross-reactions between Jin-1 and the type strains of L. pneumophila serogroup 1 through 14 were observed in both slide and tube agglutination tests mediated by the IgM antibodies. However, Jin-1 revealed the L. pneumophila serogroup 5 specificity in IFA, ELISA and dot-ELISA, all mediated by the IgG antibodies. Jin-1 was finally identified as a strain of L. pneumophila serogroup 5 with more complex antigenic composition than the ATCC type strain of this serogroup. The taxonomic conclusion has been confirmed by CDC. A Legionella pneumophila strain which serologically cross-reacting with so many serogroups of this species has never been reported in the published articles. It is presumed that the cross-reacting antigen of Jin-1 belongs to the thymus-independent antigen by nature.