| 中国人丙型肝炎病毒基因组的一级结构及其变异 |
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| 引用本文： | 毕胜利,白宪鹤.中国人丙型肝炎病毒基因组的一级结构及其变异[J].病毒学报,1993,9(2):114-127. |
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| 作者姓名： | 毕胜利 白宪鹤 |
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| 作者单位： | 中国预防医学科学院病毒学研究所，广西壮族自治区卫生防疫站，中国预防医学科学院病毒学研究所，中国预防医学科学院病毒学研究所，河北省固安县卫生防疫站，美国疾病控制中心，中国预防医学科学院病毒学研究所 北京 100052，北京 100052，北京 100052，北京 100052 |
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| 摘 要： |
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| 关 键 词： | 丙型肝炎病毒 聚合酶链反应 结构 |
PRIMARY STRUCTURE AND VARIATION OF CHINESE HEPATITIS C VIRUS GENOME |
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| Bi Shengli Bai Xianhe Cong Mianer Tian Houwen Sun Degui H.S.Margolis Liu ChongbaiInstitute of Virology,Chinese Acodemy of Preventive Medicine,Beijing,Guangxi Health and Anti-epidemic Center,Nanning,Sanitation and Anti-epidemic Station of Guan County,Hebei,Centers for Disease Control,USA.PRIMARY STRUCTURE AND VARIATION OF CHINESE HEPATITIS C VIRUS GENOME[J].Chinese Journal of Virology,1993,9(2):114-127. |
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| Authors: | Bi Shengli Bai Xianhe Cong Mianer Tian Houwen Sun Degui HSMargolis Liu ChongbaiInstitute of Virology Chinese Acodemy of Preventive Medicine Beijing Guangxi Health and Anti-epidemic Center Nanning Sanitation and Anti-epidemic Station of Guan County Hebei Centers for Disease Control USA |
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| Institution: | Bi Shengli Bai Xianhe Cong Mianer Tian Houwen Sun Degui H.S.Margolis Liu ChongbaiInstitute of Virology,Chinese Acodemy of Preventive Medicine,Beijing,100052Guangxi Health and Anti-epidemic Center,Nanning,530021Sanitation and Anti-epidemic Station of Guan County,Hebei,052700Centers for Disease Control,USA |
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| Abstract: | Two acute hepathis C patient's sera collected seperatly from Hebei Province and Shanghai in China were used for isolation of China strain hepatitis C virus ( HCV ) genome RNA. Overlapping cDNA fragments ge-nerated by PCR were sequenced with the dideoxynucleotide method. Com-paring Hebei strain HCV with the original isolates of US HCV-1 and Japan HCV-J strain , 67.6% and 91.8% homology at the nucleotide Ievel was found respectively and at the amino acid Ievel, was 84.9% and 94.3%.The results also demonstrate the heterogenicity between HCV from Hebei ( Hebei strain) and Shanghai ( Shanghai strain ) in their core and E2 regions, but not in NS3-NS4(C-100)and NS5 regions where heterogenicity was found throughout the whole genome among HCV isolates from China, Japan and the United States. |
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| Keywords: | Hepatitis C virus Polymerase chain reaction ( PCR ) Nucleotide sequence |
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