| Affiliation: | (1) Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 292-0818 Kisarazu, Chiba, Japan;(2) Department of Bioprocessing, Faculty of Agriculture, Gifu University, 501-1193 Gifu, Japan;(3) Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Kamiina, 399-4598 Nagano, Japan |
| Abstract: | Bacillus species were observed and quantified by molecular approaches, using the 165 rDNA primers/probes, in a wastewater treatment plant designed for the purpose of stimulating the growth ofBacillus species. The plant has been operating as a test plant since 1997 in the city of Ina, Japan, with excellent treatment performance. Observations byin situ hybridization, usingBacillus-specific probes, indicated thatBacillus strains were inhabited in the plant and their numbers decreased during the treatment process. Similar results were obtained from a quantitative PCR analysis using aBacillus-specific primer set, and the amount of DNA originating from variousBacillus species was maximally 1.91% of the total DNA in the wastewater treatment tank. Clone library analysis using theBacillus-specific primers suggested that, while the population was noticeably increased, the phylogenetic diversity of the increasingBacillus species was very low. |
