单质粒模式诱导表达载体系统的建立

在Invitrogen公司T-Rex诱导表达系统的基础上，将目的基因与调控蛋白基因克隆于同一载体上，并在CV1细胞中观察了四环素对该单质粒模式载体报告基因表达的诱导效应.载体在瞬时转染CV1细胞并以四环素诱导24 h后目的基因的表达水平提高了8.9倍，表明单质粒模式载体基因诱导表达的应用具有可行性.

Design and Construction of Inducible Expression Vector by Single-Plasmid Strategy

On the basis of T-Rex inducible expression system, a vector that express reporter gene and regulated gene simultaneously were constructed. The induction effects of tetracycline to the gene expression were studied in CV1 cells by transient transfection. An 8.9-fold increase in expression level of reporter gene was observed when CV1 cells, which were transiently transfected with the vector, were induced with tetracycline for 24 h, suggesting that the application of single-plasmid vecor in gene expression induction is also feasible.