Participation of macrophage membrane rafts in Trypanosoma cruzi invasion process |
| |
Authors: | Barrias E S Dutra J M F De Souza W Carvalho T M U |
| |
Affiliation: | Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, CCS, UFRJ, Ilha do Fund?o, 21941-902 Rio de Janeiro, RJ, Brazil. |
| |
Abstract: | Membrane rafts are small and dynamic regions enriched in sphingolipids, cholesterol, ganglioside GM1 and protein markers like flotillins, forming the flatter domains or caveolins, which are characterized as stable flask-shape invaginations. We explored whether membrane rafts participate in the entry of Trypanosoma cruzi's trypomastigotes into murine macrophages through transient depletion of macrophage membrane cholesterol with methyl-beta-cyclodextrin and treatment with filipin. These treatments led to a decrease in the trypomastigote invasion process. Macrophage pre incubated with increasing concentrations of cholera toxin B, that binds GM1, inhibited the adhesion and invasion of trypomastigote and amastigote forms. Immunofluorescence analysis demonstrated a colocalization of GM1, flotillin 1 and caveolin 1 in the T. cruzi parasitophorous vacuole. Taken together these data suggest that membrane rafts, including caveolae, are involved in the process of T. cruzi invasion of macrophages. |
| |
Keywords: | Trypanosoma cruzi Macrophage Caveolin 1 Flotillin 1 GM1 |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|