pH-sensitive glass microelectrodes and intracellular pH measurements |
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Authors: | Yasunobu Okada Akira Inouye |
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Institution: | 1. Department of Physiology, Kyoto University, School of Medicine, 606, Kyoto, Japan
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Abstract: | 1. |
Some properties of the open-tipped, uninsulated, pH-sensitive glass microelectrode were examined in several electrical experiments.
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Based on these observations, technical and theoretical problems were considered for application to the pH measurement in small cells.
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The intracellular pH, (pH)i, of the epithelial cell in rat duodenum measured was approximately 7.0. A reduction in (pH)i was apparent (about 0.3) with the addition of 20 mM-glucose to the bathing fluid.
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It was concluded, that with certain limitations such uninsulated, open-tipped microelectrodes may be successfully utilized for intracellular pH measurements.
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