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Assignment of the 31P and 1H resonances in oligonucleotides by two-dimensional NMR spectroscopy |
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| Authors: | V Sklenár H Miyashiro G Zon H T Miles A Bax |
| Affiliation: | 1. Laboratory of Chemical Physics Bethesda, MD 20892, USA;2. Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health Bethesda, MD 20892, USA;3. Center for Drugs and Biologics, Food and Drug Administration, Bethesda, MD 20892, USA |
| Abstract: | The use of new 1H-detected heteronuclear 1H-31P shift correlation experiments is demonstrated for oligonucleotides of 12 and 40 base pairs. The methods give unambiguous assignments of the 31P resonances and also permit identification of the C4' and C5' sugar protons. Use of the new methods enables one to make sequence-specific resonance assignments without reference to a known or assumed conformation of the DNA fragment. |
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