High level expression of a thermostable Bacillus xylose (glucose) isomerase in Escherichia coli |
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| Authors: | Liao Wuxiang Kandiah Jeyaseelan |
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| Affiliation: | (1) Department of Biochemistry, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, 0511 Singapore, Singapore |
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| Abstract: | Summary A thermophilic Bacillus sp. producing xylose (glucose) isomerase has been isolated. Its xy/A gene when cloned in Escherichia coli and expressed gave 37.5 and 12.8 units/ mg protein respectively for xylose and glucose isomerase activities at 85°C. A single heat treatment of the crude extract purified the enzyme further yielding the highest ever recorded activities of 150 and 49.02 units /mg protein. |
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