Genome and Chromosome Dimensions of Lotus japonicus |
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| Authors: | Mikako Ito Junko Miyamoto Yumiko Mori Satoru Fujimoto Toshiki Uchiumi Mikiko Abe Akihiro Suzuki Satoshi Tabata Kiichi Fukui |
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| Institution: | (1) Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, 565–0871 Japan, JP;(2) Department of Earth and Environmental Sciences, Faculty of Science, Kagoshima University, Kagoshima, 890–0065 Japan, JP;(3) Department of Chemistry and Bioscience, Faculty of Science, Kagoshima University, Kagoshima, 890–0065 Japan, JP;(4) Kazusa DNA Research Institute, Chiba, 292–0812 Japan, JP |
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| Abstract: | Lotus Japonicus , Miyakojima MG-20 and Gifu B-129. The genome sizes of Miyakojima and Gifu were determined as 472.1 and 442.8 Mbp, respectively.
Both the accessions were diploid (2n=12) and six chromosomes were identified and characterized based on the condensation patterns and the locations of rDNA loci.
The obvious polymorphism observed in the genome size and the chromosome morphology between the two accessions, revealed specific
accumulation of heterochromatin in Miyakojima or elimination in Gifu. The chromosomes L. japonicus were numbered according to their length. A quantitative chromosome map was also developed by the imaging methods using the
digital data of the condensation pattern. 45S rDNA loci were localized on chromosomes A and F, and 5S rDNA locus was localized
on chromosome A by fluorescence in situ hybridization (FISH). Identification of the chromosome and genome sizes and development of the quantitative chromosome map
represent significant contribution to the L. japonicus genome project as the basic information.
Received 29 August 2000/ Accepted in revised form 17 October 2000 |
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| Keywords: | : Condensation pattern Fluorescence in situ hybridization (FISH) Genome size Lotus japonicus Quantitative chromosome map rDNAs |
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