Interrelationships of sodium transport and carbon dioxide production by the toad bladder: Response to changes in mucosal sodium concentration,to vasopressin and to availability of metabolic substrate |
| |
Authors: | Norman S Coplon Roderic E Steele Roy H Maffly |
| |
Institution: | (1) Veterans Administration Hospital, Stanford University School of Medicine, 94304 Palo Alto, California;(2) Department of Medicine, Stanford University School of Medicine, 94304 Palo Alto, California;(3) Present address: Laboratory of Technical Development, National Heart and Lung Institute, 20014 Bethesda, Maryland |
| |
Abstract: | Summary Active sodium transport and CO2 production were measured simultaneously in toad bladders mounted in membrane chambers. The rate of sodium transport was varied by changing the concentration of sodium in the mucosal bath (substitution with choline), by adding vasopressin, by adding metabolic substrates and by adding malonate, and the ratio of the change of sodium transport and CO2 production was determined Mean values for Na/ CO2 (equiv/mole) were: Na choline 18.3±1.1; vasopressin 15.5±2.8; and pyruvate (corrected for the increment in nontransport CO2) 15.4±3.5. Based on previously determined values for the respiratory quotient (R.Q.), calculated mean values for Na/ O2 ranged between 15.5 and 18.5 equiv/mole. It appears that basal metabolism does not contribute to metabolism supporting sodium transport when the rate of sodium transport is varied. Transport metabolism appears much more responsive to changes in the availability of endogenous and exogenous substrates than does nontransport metabolism. We conclude that transport and nontransport metabolism are functionally separated in the toad bladder.These results were presented in part at the Annual Meeting of the American Society of Nephrology, November 1973. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|