Generation of Cas9 transgenic zebrafish and their application in establishing an ERV-deficient animal model |
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| Authors: | Zhe Yang author-information" >,Shihao Chen,Songlei Xue,Xinxiu Li,Zhen Sun,Yu Yang,Xuming Hu,Tuoyu Geng,Hengmi Cui |
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| Affiliation: | 1.Institute of Epigenetics and Epigenomics,Yangzhou University,Yangzhou,China;2.College of Animal Science and Technology,Yangzhou University,Yangzhou,China;3.Institute of Comparative Medicine,Yangzhou University,Yangzhou,China;4.Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou University,Yangzhou,China;5.Joint International Research Laboratory of Agricultural & Agri-Product Safety, The Ministry of Education of China,Yangzhou University,Yangzhou,China;6.College of Animal Science and Technology,Yangzhou University,Yangzhou,China |
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| Abstract: | ObjectivesTo investigate the effect of endogenous Cas9 on genome editing efficiency in transgenic zebrafish.ResultsHere we have constructed a transgenic zebrafish strain that can be screened by pigment deficiency. Compared with the traditional CRISPR injection method, the transgenic zebrafish can improve the efficiency of genome editing significantly. At the same time, we first observed that the phenotype of vertebral malformation in early embryonic development of zebrafish after ZFERV knockout.ConclusionsThe transgenic zebrafish with expressed Cas9, is more efficient in genome editing. And the results of ZFERV knockout indicated that ERV may affect the vertebral development by Notch1/Delta D signal pathway. |
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