Caenorhabditis briggsae and C. elegans: partial characterization of cuticle surface carbohydrates. |
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Authors: | B M Zuckerman I Kahane S Himmelhoch |
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Affiliation: | 1. Laboratory of Experimental Biology, College of Food and Natural Resources, University of Massachusetts, East Wareham, Massachusetts 02538 U.S.A.;2. Biomembrane Research Laboratory, Hebrew University Hadassah Medical School, Jerusalem, Israel;3. Section of Biological Ultrastructure, Weizmann Institute, Rehovot, Israel |
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Abstract: | The presence of galactose, glucose, mannose, and N-acetylglucosamine on the exposed surface of the nematodes Caenorhabditis briggsae and C. elegans was indicated by specific binding of three iodinated plant lectins. Proteolysis experiments suggested the absence of digestible glycoproteins on the exposed surfaces of the two nematode species. High resolution micrographs of cuticle surface preparations labeled with cationized ferritin indicated that the negative charge-bearing molecules are more densely packed on the nematode surface than on animal plasma membranes. |
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Keywords: | Nematodes Negative surface charge Surface carbohydrate Plant lectin Membranes |
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