| 微卫星DNA标记技术及其在植物研究中的应用 |
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| 引用本文： | 姜立春,彭正松,阮期平.微卫星DNA标记技术及其在植物研究中的应用[J].生物技术通讯,2006,17(4):654-657. |
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| 作者姓名： | 姜立春 彭正松 阮期平 |
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| 作者单位： | 1. 西华师范大学,生命科学学院,四川,南充,637002
2. 绵阳师范学院,分子生物学与生物制药重点实验室,四川,绵阳,621000 |
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| 摘 要： | 微卫星DNA(或简单重复序列,simplesequencerepeats,SSR)是继RFLP、RAPD等分子标记后出现的第二代分子标记技术。随着分子生物学的发展,微卫星标记技术在植物基因组中的应用越来越广泛。由于SSR具有多态性高,呈共显性遗传,遵守孟德尔式分离,在数量上没有生物学的限制,实验操作简单,对样品质量要求不高等特点,因此被广泛用于植物遗传图谱的构建、基因定位、构建指纹图谱、遗传多样性及物种进化与亲缘关系的研究等方面。
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| 关 键 词： | 微卫星DNA 简单重复序列 遗传图谱 分子标记 遗传多样性 基因定位 |
| 文章编号： | 1009-0002(2006)04-0654-04 |
| 收稿时间： | 10 9 2005 12:00AM |
| 修稿时间： | 2005年10月9日 |
Microsatellites DNA Markers and Their Application in Research of Plants |
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| JIANG Li-chun,PENG Zheng-song,RUAN Qi-ping.Microsatellites DNA Markers and Their Application in Research of Plants[J].Letters in Biotechnology,2006,17(4):654-657. |
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| Authors: | JIANG Li-chun PENG Zheng-song RUAN Qi-ping |
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| Institution: | 1. College of Life Science, China West Normal University, Nanchong 637002; 2. Key Laboratory for Morlecular Biology and Biopharmaceuticals, Mianyang Normal University, Mianyang 621000; china |
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| Abstract: | Microsatellites DNA(or simple sequence repeats,SSR) are tandem repeat of short uncleotide motifs dispersing widely and relatively evenly in eukaryotic genomes.It is the second molecular marker technology after RFLP and RAPD.Its principle and characteristic were discussed in this paper.As a highly polymorphic,codominant,well-repeated,no biologically restrictive in quantity and convenient molecular marker technique,SSR markers tequnique was applied by the construction of plant molecular genetic linkage map,identification of gene or QTL,evolution and classfication of plant species,genetic diversity,identification and purity of varieties in plants. |
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| Keywords: | microsatellites DNA simple sequence repeats genetic mapping molecular marker genetic diversity gene location |
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