| 产环己酰亚胺菌株YIM41004T发酵条件的研究 |
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| 引用本文： | 夏振远,郭端强,李铭刚,陈义光,李一青,文孟良. 产环己酰亚胺菌株YIM41004T发酵条件的研究[J]. 工业微生物, 2005, 35(2): 37-40 |
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| 作者姓名： | 夏振远 郭端强 李铭刚 陈义光 李一青 文孟良 |
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| 作者单位： | 云南省烟草科学研究所,玉溪,653100;云南大学省微生物研究所教育部微生物资源重点实验室,昆明,650091;吉首大学生命科学学院,吉首,416000 |
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| 基金项目： | 云南省烟草公司基金资助(04A16) |
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| 摘 要： | 以产环已酰亚胺菌株链霉菌(Streptomyces yunnanensis)YIM41004T为研究对象,对其发酵培养基和发酵条件进行了优化研究,得到的最佳培养基组成为葡萄糖6%,大豆粉1%,硫酸铵0.5%,蛋白胨0.2%,碳酸钙0.6%,硫酸镁0.05%,磷酸二氢钾O.02%;优化后的培养条件为以4%接种量接种至500mL三角瓶中,装液量为75 mL,初始pH值6.5,发酵温度为28℃,摇床培养96 h.优化后环己酰亚胺产量平均达到445.19 ug/mL,比初始的环己酰亚胺产量提高了422%(p＜0.01).
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| 关 键 词： | 云南链霉菌 环已酰亚胺 发酵条件 |
Fermentation conditions for cycloheximide producing strain YIM41004T |
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| XIA Zhen-yuan,GUO Duan-Qiang,LI Ming-Gang,CHEN Yi-guang,LI Yi-qing,WEN Men-Liang. Fermentation conditions for cycloheximide producing strain YIM41004T[J]. Industrial Microbiology, 2005, 35(2): 37-40 |
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| Authors: | XIA Zhen-yuan GUO Duan-Qiang LI Ming-Gang CHEN Yi-guang LI Yi-qing WEN Men-Liang |
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| Affiliation: | XIA Zhen-Yuan~1,GUO Duan-Qiang~2,LI Ming-Gang~2,CHEN Yi-Guang~3,LI Yi-qing~2,WEN Men-Liang~ |
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| Abstract: | Fermentation conditions of liquid culture for the cycloheximide producing strain YIM41004~T from Streptomyces yunnanensis were studied. The results indicated that the optimum medium and optimized conditions were as follows: glucose 6%, soybean cake 1%, (NH_4)_2SO_4 0.5%, protose peptone 0.2%, CaCO_3 0.6%, MgSO4 0.05%, KH_2PO_4 0.02%; inoculation volume 4% for 75 mL fermentation medium in 500 mL flask, initial pH6.5, cultivate temperature 28 and cultivation period 96 h.. The cycloheximide productivity of fermentation fluid reached 445.19 g/mL, increased by 422% if compared with that of the original(p<0.01). |
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| Keywords: | Streptomyces yunnanensis cycloheximide fermentation conditions |
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