Cell cycle effects in yeast cells of the antifungal agent Sinefungin

Abstract The S -adenosylmethionine (AdoMet) analog Sinefungin (SF) caused actively dividing cells of the yeast Saccharomyces cerevisiae to arrest within one cell cycle as unbudded cells. Reciprocal shift experiments showed that these cells were blocked in performance of the cell cycle regulatory step "start". Both protein and RNA synthesis rates were only moderately affected during SF-mediated cell cycle arrest; these results indicate that SF provokes a different sort of metabolic response than found upon treatment with other "start"-arrest compounds.