Thiamine increases β-glucosidase production in the newly isolated strain of Fomitopsis pinicola |
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Authors: | A-R Joo K-M Lee W-I Sim M Jeya M-R Hong Y-S Kim D-K Oh J-K Lee |
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Institution: | Department of Bioscience and Biotechnology, Konkuk University, Seoul, Korea; Department of Chemical Engineering, Konkuk University, Seoul, Korea; Institute of Biomedical Science and Technology, Konkuk University, Seoul, Korea; Department of Forest Products, Kookmin University, Seoul, Korea |
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Abstract: | Aims: To isolate a high β-glucosidase (BGL)-producing strain and to optimize BGL production in the isolated strain. Methods and Results: A high BGL-producing strain was isolated and identified as Fomitopsis pinicola KMJ812 based on its morphology and a comparison of sequence of its internal transcribed spacer rDNA gene. To increase BGL production, F. pinicola was supplemented with various vitamins. Supplementation with thiamine (20 mg l?1) improved BGL production in F. pinicola cultures by 3·7-fold to give a specific activity of 114·4 μmol min?1 mg?1 protein, one of the highest among BGL-producing micro-organisms. The increased production of BGL in the thiamine-supplemented culture was confirmed by 2D electrophoresis followed by MS/MS sequencing. The BGL purified from F. pinicola culture showed the highest catalytic efficiency ever reported. Conclusion: Supplemental thiamine remarkably increased BGL production by a novel BGL-producing strain, F. pinicola KMJ812. Significance and Impact of the Study: Our results provide a high BGL-producing strain and the production media for BGL production, and should contribute to better industrial production of glucose via biological processes. |
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Keywords: | Fomitopsis pinicola specific activity thiamine vitamin β-glucosidase |
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