Identification of DNA ligase I related polypeptides in three different human cells |
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| Authors: | C Prigent S Aoufouchi M Philippe |
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| Institution: | Laboratoire de Biologie et Génétique du Développement URA CNRS 256, Université de Rennes I, France. |
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| Abstract: | Partial purification of the DNA ligase from three human tissues (liver, thymus and lymphoblasts) revealed that each cell type contains several different polypeptides bearing a DNA ligase I activity. Their apparent molecular weights estimated after SDS PAGE, 130 kDa, 100 kDa and 80 kDa, are in agreement with previous reports. These polypeptides are related by proteolysis to a single higher molecular weight protein of 200 kDa which does not show DNA ligase activity but that could be a preprotein. |
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