Oral immunogenicity and protective efficacy in mice of transgenic rice plants producing a vaccine candidate antigen (As16) of Ascaris suum fused with cholera toxin B subunit |
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| Authors: | Yasunobu Matsumoto Seiko Suzuki Tomoko Nozoye Takashi Yamakawa Yasuhiro Takashima Takeshi Arakawa Naotoshi Tsuji Fumio Takaiwa Yoshihiro Hayashi |
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| Institution: | (1) Laboratory of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;(2) Laboratory of Plant Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;(3) Department of Veterinary Parasitological Diseases, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagito, 501-1193 Gifu, Japan;(4) Molecular Microbiology Group, Center of Molecular Biosciences, University of the Ryukyus, Nishihara Okinawa, 903-0213, Japan;(5) Laboratory of Parasitic Diseases, National Institute of Animal Health, National Agricultural Research Organization, Tsukuba Ibaraki, 305-0856, Japan;(6) Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba Ibaraki, 305-8602, Japan |
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| Abstract: | Cereal crops such as maize and rice are considered attractive for vaccine production and oral delivery. Here, we evaluated
the rice Oryza sativa for production of As16—an antigen protective against the roundworm Ascaris suum. The antigen was produced as a chimeric protein fused with cholera toxin B subunit (CTB), and its expression level in the
endosperm reached 50 μg/g seed. Feeding the transgenic (Tg) rice seeds to mice elicited an As16-specific serum antibody response
when administered in combination with cholera toxin (CT) as the mucosal adjuvant. Although omitting the adjuvant from the
vaccine formulation resulted in failure to develop the specific immune response, subcutaneous booster immunization with bacterially
expressed As16 induced the antibody response, indicating priming capability of the Tg rice. Tg rice/CT-fed mice orally administered
A. suum eggs had a lower lung worm burden than control mice. This suggests that the rice-delivered antigen functions as a prophylactic
edible vaccine for controlling parasitic infection in animals. |
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| Keywords: | Transgenic rice Oral vaccine Ascaris suum Cholera toxin B subunit Mucosal adjuvant |
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