Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring |
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| Authors: | Maxwell T Koobatian Carmon Koenigsknecht Sindhu Row Stelios Andreadis Daniel Swartz |
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| Institution: | 1.Department of Physiology & Bio-Physics, State University of New York Buffalo School of Medicine;2.Department of Pediatrics, State University of New York Buffalo School of Medicine;3.Department of Chemical and Biological Engineering, State University of New York Buffalo School of Engineering |
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| Abstract: | The development of Tissue Engineered Vessels (TEVs) is advanced by the ability to routinely and effectively implant TEVs (4-5 mm in diameter) into a large animal model. A step by-step protocol for inter-positional placement of the TEV and real-time digital assessment of the TEV and native carotid arteries is described here. In vivo monitoring is made possible by the implantation of flow probes, catheters and ultrasonic crystals (capable of recording dynamic diameter changes of implanted TEVs and native carotid arteries) at the time of surgery. Once implanted, researchers can calculate arterial blood flow patterns, invasive blood pressure and artery diameter yielding parameters such as pulse wave velocity, augmentation index, pulse pressures and compliance. Data acquisition is accomplished using a single computer program for analysis throughout the duration of the experiment. Such invaluable data provides insight into TEV matrix remodeling, its resemblance to native/sham controls and overall TEV performance in vivo. |
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| Keywords: | Bioengineering Issue 98 Vascular surgery Tissue Engineered Vessel Surgical Technique Bio-Engineering Vascular Grafts Implantation Sheep Large animal model Carotid Artery Anastomosis |
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