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Use of endogenous signal sequences for transient production and efficient secretion by moss (<Emphasis Type="Italic">Physcomitrella patens</Emphasis>) cells |
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| Authors: | Andreas?Schaaf Stefanie?Tintelnot Armin?Baur Ralf?Reski Gilbert?Gorr |
| Institution: | 1.Department of Plant Biotechnology, Faculty of Biology,University of Freiburg,Freiburg,Germany;2.greenovation Biotech GmbH,Freiburg,Germany;3.Department of Plant Biochemistry and Biotechnology,University of Münster,Münster,Germany |
| Abstract: | BackgroundEfficient targeting to appropriate cell organelles is one of the bottlenecks for the production of recombinant proteins in plant systems. A common practice is to use the native secretory signal peptide of the heterologous protein to be produced. Though general features of secretion signals are conserved between plants and animals, the broad sequence variability among signal peptides suggests differing efficiency of signal peptide recognition. |
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