Use of endogenous signal sequences for transient production and efficient secretion by moss (<Emphasis Type="Italic">Physcomitrella patens</Emphasis>) cells
1.Department of Plant Biotechnology, Faculty of Biology,University of Freiburg,Freiburg,Germany;2.greenovation Biotech GmbH,Freiburg,Germany;3.Department of Plant Biochemistry and Biotechnology,University of Münster,Münster,Germany
Abstract:
Background
Efficient targeting to appropriate cell organelles is one of the bottlenecks for the production of recombinant proteins in
plant systems. A common practice is to use the native secretory signal peptide of the heterologous protein to be produced.
Though general features of secretion signals are conserved between plants and animals, the broad sequence variability among
signal peptides suggests differing efficiency of signal peptide recognition.