新疆阿克苏地区盐碱地粘细菌分离鉴定

【目的】采用传统的纯培养技术,分离新疆阿克苏地区典型的盐碱地中的粘细菌,并初步分析盐碱地土壤中可培养粘细菌资源的多样性。【方法】采用传统的水琼脂法、滤纸法和改良的土壤浸出液法分离新疆阿克苏地区25份盐碱地的粘细菌。结合分析土样的酸碱度、含盐量、地理位置及其植被分布情况分析新疆阿克苏地区盐碱地粘细菌资源多样性。【结果】共分离到58株粘细菌,它们被鉴定为:粘球菌属(Myxococcus)33株;珊瑚球菌属(Corallococcus)14株;孢囊杆菌属(Cystobacter)6株;堆囊菌属(Sorangium)2株;侏囊菌属(Nannocystis)2株;多囊菌属(Polyangium)1株。其中粘球菌抗逆性强,分离的菌株数最多,在pH值7.5-8.5范围的盐碱地中普遍存在;其次为珊瑚球菌属;而侏囊菌属、多囊菌属的菌株较少见。【结论】新疆阿克苏地区盐碱地粘细菌多样性不高,可能受分离纯化方法、含盐量以及土壤性质影响较大。

Isolation and identification of myxobacteria in the salinealkaline soils of Akesu in Xinjiang

[Objective]To isolate myxobacteria and investigate their diversity in saline-alkaline soils from Akesu in Xinjiang.[Methods]Conventional culture-dependent methods,e.g.baiting technique,water agar,soil extract agar and mineral agar,were used to isolate myxobacteria from 25 soil samples collected from Akesu areas of Xinjiang.Combining with physicochemical properties(acidity/alkalinity,salt concentration,vegetation and geographical locations) of the soil samples,myxobacterial diversity was studied.[Results]In total 58 strains were isolated,and identified as belonging to 6 different genera,i.e.Myxococcus,Cystobacter,Corallococcus,Sorangium,Nannocystis and Polyangium of Myxococcales.The most frequent genus isolated was Myxococcus which may better adapt in harsh environments.Different myxobacterial diversity was detected in different habitat.[Conclusion]Myxobacteria diversity was low in saline-alkaline soils of Akesu in Xinjiang.