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Identification of the Cloned Gene for the Murine Transplantation Antigen H-2Kb by Hybridization with Synthetic Oligonucleotides |
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| Authors: | Dan H Schulze Larry R Pease Yuichi Obata Stanley G Nathenson Antonio A Reyes Satoshi Ikuta and R Bruce Wallace |
| Institution: | 1Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461;2Memorial Sloan-Kettering Cancer Center, New York, New York 10021;3Department of Molecular Genetics, City of Hope Research Institute, Duarte, California 91010 |
| Abstract: | The H-2Kb gene is a member of the large major histocompatibility complex class I gene family. Since many members of this family cross-hybridize with class I cDNA probes, the cloned H-2Kb gene was identified by hybridization with specific oligonucleotide probes. This clone was definitively shown to encode the H-2Kb polypeptide by partial DNA sequencing and by serological and tryptic peptide analyses of the expressed product. |
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