Effect of glycl-tRNA concentration on in vitro serine incorporation into the peptidoglycan of S. epidermidis |
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Authors: | R H Hilderman H G Riggs |
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Institution: | Department of Microbiology, University of Missouri School of Medicine, Columbia, Missouri 65201 USA |
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Abstract: | Seven species of seryl-tRNA have been isolated from strain Texas 26 by the use of Plaskon CTFE powder as the support for Adogen 464 in reversed-phase chromatography. All but one of these seven seryl-tRNA incorporated 14C-serine into biosynthesized peptidoglycan. This suggested that at least one of the functional roles of six of the seven seryl-tRNA peaks isolated may be involved in cell wall synthesis. It has also been shown that the ratio of glycine to serine in the pentapeptide bridge depended upon the concentration of glycyl-tRNA in the assay mixture. By increasing the concentration of glycyl-tRNA the amount of serine inserted into the bridge is increased. Thus the amount of serine incorporated into the pentapeptide bridge appears to be dependent upon the concentration of glycyl-tRNA. |
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