| 利用Cyt b 基因由未知鲸骨鉴定出大村鲸 |
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| 引用本文： | 马牧,祝茜,李响,孙玉苗,刘莹莹,刘亚楠,陶翠花.利用Cyt b 基因由未知鲸骨鉴定出大村鲸[J].兽类学报,2007,27(3):288-292. |
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| 作者姓名： | 马牧 祝茜 李响 孙玉苗 刘莹莹 刘亚楠 陶翠花 |
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| 作者单位： | 山东大学威海分校海洋学院, 威海 |
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| 基金项目： | 国家自然科学基金;中华人民共和国农业部资助项目;山东大学校科研和教改项目 |
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| 摘 要： | 作者于2005年2月从广东省湛江市硇洲岛一位渔民手中采集到一块鲸类的脊椎骨,据渔民对该搁浅鲸类的外形描述推断该骨骼属于一种大型须鲸.为了鉴定该脊椎骨所属的种类,本文利用分子生物学技术,从中提取基因组DNA,并以此为模板特异性扩增Cyt b基因的部分序列进行物种鉴定.
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| 关 键 词： | 鲸类脊椎骨 细胞色素b基因 分子进化树 种类鉴定 大村鲸 |
| 文章编号： | 1000-1050（2007）03-0288-05 |
| 收稿时间： | 2006-10-30 |
| 修稿时间： | 2007-05-23 |
Identification of Balaenoptera omurai from an unknown whale vertebra based on Cyt b gene sequence |
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| MA Mu,ZHU Qian,LI Xiang,SUN Yumiao,LIU Yingying,LIU Yanan,TAO Cuihua.Identification of Balaenoptera omurai from an unknown whale vertebra based on Cyt b gene sequence[J].Acta Theriologica Sinica,2007,27(3):288-292. |
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| Authors: | MA Mu ZHU Qian LI Xiang SUN Yumiao LIU Yingying LIU Yanan TAO Cuihua |
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| Institution: | Ocean College,Shandong University at Weihai,Weihai 264209,China |
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| Abstract: | In order to identify an unknown whale vertebra's species,genomic DNA is extracted from the bone powder by proteinase K and phenol /chloroform,and a partial Cyt b gene sequence as long as 565 bp is amplified by PCR (Polymerase Chain Reaction). The blasting result in GenBank shows that the vertebra sample has maximal similarity with Balaenoptera omurai, it is 99%. It indicates that the vertebra is Balaenoptera omurai. The phylogenetic tree based on UPGMA is consistent with the blasting result. Analysis of software MEGA shows that the Cyt b gene sequence of the vertebra sample has 1 -2 nucleotides which are different from the homologous sequence of B. omurai (accession AB201257,AB201256), thus, the sequence is a new haplotype This is the first record of B. omurai which distributes in the South China Sea. |
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| Keywords: | Balaenoptera omurai Cytochrome b gene Phylogenetic tree Species identification Whale vertebra |
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