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A genome-scale gain-of-function CRISPR screen in CD8 T cells identifies proline metabolism as a means to enhance CAR-T therapy
Institution:1. System Biology Institute, Integrated Science & Technology Center, West Haven, CT 06516, USA;2. Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA;3. Center for Cancer Systems Biology, Integrated Science & Technology Center, West Haven, CT 06516, USA;4. Yale M.D.-Ph.D. Program, 367 Cedar Street, New Haven, CT 06510, USA;5. Combined Program in the Biological and Biomedical Sciences, Yale University, New Haven, CT 06510, USA;6. MCGD Program, Yale University, New Haven, CT 06510, USA;7. Immunobiology Program, Yale University, New Haven, CT 06520, USA;8. Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA;9. The College, Yale University, New Haven, CT 06520, USA;10. Advanced Cell Therapy Laboratory, Yale University School of Medicine, New Haven, CT 06520, USA;11. Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520, USA;12. Department of Epidemiology of Microbial Diseases, Yale University School of Public Health, New Haven, CT 06520, USA;13. Department of Pathology, Yale University School of Medicine, New Haven, CT 06520, USA;14. Department of Rheumatology, Yale University School of Medicine, New Haven, CT 06520, USA;15. Center for Biomedical Data Science, Yale University School of Medicine, New Haven, CT, USA;16. Comprehensive Cancer Center, Yale University, New Haven, CT 06510, USA;17. Department of Neurosurgery, Yale University School of Medicine, New Haven, CT, USA;18. Yale Stem Cell Center, Yale University School of Medicine, New Haven, CT, USA;19. Yale Liver Center, Yale University School of Medicine, New Haven, CT, USA;20. Center for RNA Science and Medicine, Yale University, New Haven, CT 06510, USA
Abstract:
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  • Keywords:CAR-T  PRODH2  T cell GOF screen  T cell CRISPR activation screen  metabolism  proline metabolism  dead-guide RNA  antitumor efficacy  mitochondria
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