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Production of catechols and muconic acids from various aromatics by the styrene-degraderRhodococcus rhodochrous NCIMB 13259 |
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| Authors: | A. Michael Warhurst Kenneth F. Clarke Robert A. Hill Robert A. Holt Charles A. Fewson |
| Affiliation: | (1) Department of Biochemistry, University of Glasgow, G12 8QQ Glasgow, Scotland, UK;(2) Department of Chemistry, University of Glasgow, G12 8QQ Glasgow, Scotland, UK;(3) BCRA Scientific and Technical Services Ltd., Mill Lane, Wingerworth, S42 6NG Chesterfield, Derbyshire, UK;(4) Zeneca Bio Products, Billingham, PO Box 2, TS23 1YN Cleveland, UK |
| Abstract: | Summary Intact cells ofRhodococcus rhodochrous NCIMB 13259 which had been grown in the presence of styrene were used to produce metabolic intermediates from other aromatic substrates in the presence of 3-fluorocatechol as an inhibitor of catechol oxygenase. Toluene and ethylbenzene gave 3-methylcatechol and 3-ethylcatechol respectively. The enol form of 3-acetylcatechol was produced from acetophenone and 1-phenylethanol. Benzoic acid was produced from cinnamic acid. This organism cannot metabolise muconic acids, and methylmuconic acid and ethylmuconic acid accumulated under appropriate conditions. 2-Fluoromuconic acid was also produced from the added 3-fluorocatechol. This organism provides a convenient tool for producing catechols and muconic acids from a variety of substrates without need for gentic manipulation. |
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