Mitogenic response to epidermal growth factor (EGF) modulated by platelet-derived growth factor in cultured fibroblasts |
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| Authors: | Walker Wharton Edward Leof Nancy Olashaw E. J. O''Keefe W. J. Pledger |
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| Affiliation: | 1. Department of Pharmacology, Cancer Research Center, University of North Carolina, School of Medicine, Chapel Hill, NC 27514, USA;2. Department of Bacteriology and Immunology, Cancer Research Center, University of North Carolina, School of Medicine, Chapel Hill, NC 27514, USA;3. Department of Dermatology and Cancer Cell Biology Program, Cancer Research Center, University of North Carolina, School of Medicine, Chapel Hill, NC 27514, USA |
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| Abstract: | The mitotic effects of epidermal growth factor (EGF) were investigated in two cultured fibroblast lines, BALB/c-3T3 and C3H 10T1/2 cells. EGF (30 ng/ml) added to quiescent 3T3 cells in medium containing either platelet-poor plasma or 10(-5) M insulin caused only minimal increases in the percentage of cells stimulated to initiate DNA synthesis. In contrast, EGF acted synergistically with either insulin or plasma to stimulate DNA synthesis in quiescent cultures of 10T1/2 cells, although the maximum effects of EGF were measured at concentrations several-fold greater than those found in either serum or plasma. In either 3T3 or 10T1/2 cells a transient preexposure to platelet-derived growth factor (PDGF) caused over a 10-fold increase in the sensitivity to the mitogenic effects of EGF. It is therefore possible that a primary action of PDGF is to increase the sensitivity of fibroblasts to EGF, independent of whether EGF alone is found to be mitogenic. |
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| Keywords: | To whom reprint requests should be sent. Address: Experimental Pathology Group Mail Stop M888 University of California Los Alamos National Laboratory Los Alamos NM 87545 USA. |
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