Development of a sequence characterized amplified region (SCAR) marker associated with high rooting ability in Larix |
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| Authors: | H. Li S. G. Zhang J. M. Gao C. G. Wang Y. Zhang L. W. Qi L. Chen W. Q. Song |
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| Affiliation: | (1) Laboratory of Cell Biology, College of Life Sciences, Nankai University, Tianjin, 300071, P.R. China;(2) Department of Horticulture, Tianjin Agricultural University, Tianjin, 300384, P.R. China;(3) Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, P.R. China;(4) Department of Agriculture, Tianjin Agricultural University, Tianjin, 300384, P.R. China;(5) University of Electronic Science and Technology of China, Chendu, 610054, P.R. China |
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| Abstract: | In this study, bulked segregant analysis (BSA) was used on Larix leptolepis × Larix olgensis hybrids to identify a random amplified polymorphic DNA (RAPD) marker associated with high rooting ability in larch. Two DNA bulks: H (high rooting ability) bulk and L (low rooting ability) bulk were constructed according to the rooting percentages of the stock plants. Among the 328 primers, only S356 could amplify a specific band, named S356445, which only existed in the H bulk and was further confirmed following selective genotyping of individual hybrids. Grounded on the border sequences, S356445 was converted to a sequence characterized amplified region (SCAR) marker, HRL445, which can be useful in marker-assisted selection (MAS) to screen for larch with high rooting ability. All the results strongly indicated that S356445 and HRL445 were closely associated with high rooting ability in larch. |
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| Keywords: | KeywordHeading" >Additional BSA hybrid MAS RAPD rooting percentages |
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