| 乙型肝炎病毒adr亚型NC-1毒株表面抗原基因的顺序测定与结构研究 |
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| 引用本文： | 琦祖和,阎珺,熊伟军,蔡良琬.乙型肝炎病毒adr亚型NC-1毒株表面抗原基因的顺序测定与结构研究[J].中国生物化学与分子生物学报,1988,4(3):201-209. |
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| 作者姓名： | 琦祖和 阎珺 熊伟军 蔡良琬 |
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| 作者单位： | 中国协和医科大学基础部，中国协和医科大学基础部，中国协和医科大学基础部，中国协和医科大学基础部 中国医学科学院基础医学研究所生化室，北京，中国医学科学院基础医学研究所生化室，北京，中国医学科学院基础医学研究所生化室，北京，中国医学科学院基础医学研究所生化室，北京 |
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| 摘 要： | 用内引物法自pHBVNC-1质粒DNA经Sau3A1降解的1.3Kb片段中,快速、连续测定了HBV adr NC-1表面抗原基因全顺序,与其它三株adr亚型S基因比较,顺序同源性为99％,与adw及ayw亚型比较,同源性为94％。不同亚型间的错义突变比同一亚型不同毒株间的错义突变多。比较11株adr亚型、2抹adw亚型与2株ayw亚型的S基因全顺序,发现在第47,110,113,126,160位的密码子在r亚型中有同源性,在w亚型中也有同源性,所以是w/r亚型决定簇的候选部位。第46,68,134,159,168位的密码子在d亚型中有同源性,而在y亚型中也有同源性,所以是d/y亚型抗原决定簇的候选部位。
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| 关 键 词： | 乙型肝炎表面抗原 内引物 同源性 亚型抗原决定簇 |
| 收稿时间： | 1988-06-20 |
DETERMINATION OF THE NUCLEOTIDE SEQUENCE AND STUDIES ON THE STRUCTURE OF HEPATITIS B VIRUS (HBV) adr NC-1 SURFACE ANTIGEN (HBsAg) GENE |
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| Qi,Zu-he Yan,Jim Xiong,Wei-jun Cai,Liang-wan.DETERMINATION OF THE NUCLEOTIDE SEQUENCE AND STUDIES ON THE STRUCTURE OF HEPATITIS B VIRUS (HBV) adr NC-1 SURFACE ANTIGEN (HBsAg) GENE[J].Chinese Journal of Biochemistry and Molecular Biology,1988,4(3):201-209. |
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| Authors: | Qi Zu-he Yan Jim Xiong Wei-jun Cai Liang-wan |
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| Institution: | (Dept. of Biochem., Faculty of Basic Medicine, PekingUnion Medical College)(Dept. of Biochem., Institute of Basic Medical Sciences, Chinese Acadimy of Medical Sciences |
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| Abstract: | By using internal primers the sequence of HBsAg gene of adr NC-1 has been determined. Comparison of the sequence adr NC-1 with that of the adr-pHBr330 pADR1 adr4 and adw ayw showed that the homology between different strains of adr subtype is 99% and the homology between adr NC-1 and adw or ayw subtypes is 94%. The missense mutation between adr NC-1 and adw or ayw is higher than between the different strains of adr. Comparison of the 11 strains of adr, gene, 2 strains of adw and 2 strains of ayw showed the amino acid residues in position 47, 110, 126, 160 may be regarded as the candidates of w/r determinants. And 46, 68, 134, 159, 168 are candidates of d/y determinants. |
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| Keywords: | Hepatitis B surface antigen (HBsAg) gene Internal primers Homology Determinant |
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