Quantitative determination of the major saponin mixture bacoside A in Bacopa monnieri by HPLC |
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Authors: | Deepak M Sangli G K Arun P C Amit A |
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Institution: | Department of Phytochemistry, Research and Development Centre, Natural Remedies Pvt Ltd, Veerasandra Industrial Area, Bangalore-560 100, India. phytochemistry@naturalremedy.com |
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Abstract: | Bacoside A, the putative bioactive component of the Indian medicinal plant Bacopa monnieri, was found to be a mixture of saponins with bacoside A3 (1), bacopaside II (2), jujubogenin isomer of bacopasaponin C (3) and bacopasaponin C (4) as major constituents. An HPLC method together with an optimised extraction procedure was developed for the estimation of 1-4 in B. monnieri to enable standardisation of the latter. Concentration ranges of the analytes in samples of B. monnieri collected from different regions of India were 0.14-0.85% (w/w) (1), 0.12-0.69% (2), 0.05-0.72% (3) and 0.05-0.44% (4). The importance of using bacoside A, with known concentrations of 1-4, as a reference standard for the routine analysis of B. monnieri is highlighted. Two common flavonoids, luteolin and apigenin, were present in all samples of B. monnieri. |
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Keywords: | Quantitative HPLC bacoside A bacoside A3 bacopasaponin C bacopaside II jujubogenin pseudojujubogenin Bacopa monnieri |
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