Studies on Tn10 transposition and excision in DNA-repair mutants of Salmonella typhimurium

Transposition of Tn10 in polA, recA, uvrB, mutH and uvrD mutants of Salmonella typhimurium was studied by a mating-out assay mediated by R plasmid pKM101. A decrease in transposition frequency was observed with polA, recA and uvrD mutants; uvrB and mutH mutants showed frequencies somewhat higher than control values. No effect of dimethyl sulfoxide, sodium acetate or nitrofurazone on Tn10 transposition was observed with this assay. Precise excision of Tn10 from srl202::Tn10 in these DNA-repair mutants was also studied. An increase in excision frequency of about 20 or 150 times in 2 different polA mutants, and a smaller increase, of about 2 or 15 times over control values, was detected in mutH and uvrD mutants, respectively.