Electron transfer properties of melanin. |
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Authors: | E V Gan H F Haberman I A Menon |
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Institution: | Clinical Science Division, Medical Sciences Building, University of Toronto, Toronto M5S 1A8, Canada |
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Abstract: | Melanin synthesized from mushroom tyrosinase and 3,4-dihydroxyphenylalanine has been shown to oxidize NADH and NADPH, reduce ferricyanide, oxidized forms of cytochrome c and dichlorophenolindophenol, and catalyze the coupled oxidation of NADH and reduction of ferricyanide. Kinetic studies involving the determination of initial velocity at various concentrations of substrates and product inhibition measurements have been carried out on the NADH-ferricyanide-melanin reaction. The results are consistent with a ping-pong mechanism in which one product is formed prior to the reaction of melanin with the second substrate involving the reversible oxidation and reduction of melanin during the reaction. It may be concluded that melanin is capable of acting as an electron transfer agent in several reduction-oxidation systems. |
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Keywords: | DCPIP dichlorophenolindophenol dopa L-3 4-dihydroxyphenylalanine GSH glutathione GSSG oxidized glutathione |
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