Regeneration and large-scale propagation of bamboo (Dendrocalamus strictus Nees) through somatic embryogenesis |
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Authors: | S. Saxena V. Dhawan |
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Affiliation: | (1) Tata Energy Research Institute, Darbari Seth Block, Habitat Place, Lodhi Road, New Delhi-110 003, India e-mail: sanjays@teri.res.in Fax: +91-4621770, IN |
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Abstract: | A complete protocol for large-scale propagation of Dendrocalamus strictus Nees by somatic embryogenesis has been developed. Seeds cultured on agar-solidified Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D; 3×10–5 m) produced embryogenic callus from proliferation of the embryo. Somatic embryos formed in vitro multiplied rapidly (two- to five fold every 5 weeks) on semi-solid MS medium containing 2,4-D (1×10–5 m), kinetin (Kn) (5×10–6 m), 1-indolebutyric acid (IBA) (2×10–6 m) and soluble polyvinylpyrrolidone (PVP) (250 mg l–1), or MS with 2,4-D (1×10–5 m), 6-benzylaminopurine (BAP) (1×10–5 m), and soluble PVP (250 mg l–1). Upon transfer to MS containing 1-naphthaleneacetic acid (NAA) (5×10–6 m), Kn (5×10–6 m) and soluble PVP (250 mg l–1), the dark-green embryos developed into healthy plantlets. Unrooted shoots, if any, obtained on the multiplication media were rooted on MS major salts reduced to half strength supplemented with NAA (3×10–6 m) and IBA (2.5×10–6 m). The rooted plants were successfully transferred to soil in polythene bags with over 80% survival. Using this methodology, more than 100,000 plants have been produced. Received: 16 April 1998 / Revision received: 25 September 1998 / Accepted: 10 October 1998 |
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Keywords: | Bamboo Dendrocalamus strictus Large-scale propagation Somatic embryogenesis |
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