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Optimization of laser capture microdissection and RNA amplification for gene expression profiling of prostate cancer |
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| Authors: | Dagmar M Kube Cemile D Savci-Heijink Anne-Françoise Lamblin Farhad Kosari George Vasmatzis John C Cheville Donald P Connelly George G Klee |
| Institution: | (1) Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, 200 First St. S.W., Rochester, Minnesota 55905, USA;(2) Comprehensive Cancer Center, Mayo Clinic College of Medicine, 200 First St. S.W., Rochester, Minnesota 55905, USA;(3) Department of Laboratory Medicine and Pathology, University of Minnesota, 425 Delaware St. S.E., Minneapolis, Minnesota 55455, USA;(4) Cancer Center Informatics Shared Resource, University of Minnesota, 425 Delaware St. S.E., Minneapolis, Minnesota 55455, USA;(5) Fish & Richardson P.C., 60 South Sixth Street, Minneapolis, Minnesota 55402, USA |
| Abstract: | BackgroundTo discover prostate cancer biomarkers, we profiled gene expression in benign and malignant cells laser capture microdissected (LCM) from prostate tissues and metastatic prostatic adenocarcinomas. Here we present methods developed, optimized, and validated to obtain high quality gene expression data. |
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