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Construction and evaluation of multisite recombinatorial (Gateway) cloning vectors for Gram-positive bacteria |
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| Authors: | Tania M Perehinec Saara NA Qazi Sanyasi R Gaddipati Vyvyan Salisbury Catherine ED Rees Philip J Hill |
| Affiliation: | (1) School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leics, LE12 5RD, UK;(2) Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK;(3) Faculty of Applied Sciences, University of the West of England, Bristol, UK |
| Abstract: | BackgroundThe Gateway recombinatorial cloning system allows easy and rapid joining of DNA fragments. Here we report the construction and evaluation of three different Gram-positive vectors that can be used with the Multisite Gateway cloning system to rapidly produce new gene arrangements in plasmid constructs for use in a variety of Gram-positive bacteria. |
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