Selection of reliable reference genes for qPCR studies on chondroprotective action |
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| Authors: | Stefan Toegel Wenwen Huang Claudia Piana Frank M Unger Michael Wirth Mary B Goldring Franz Gabor Helmut Viernstein |
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| Institution: | (1) Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria;(2) Beth Israel Deaconess Medical Center, New England Baptist Bone and Joint Institute, and Harvard Medical School, Boston, Massachusetts 02115, USA |
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| Abstract: | Background Chondroprotective agents (CPA) such as glucosamine, curcumin and diacerein represent potential remedies for the management
of osteoarthritis and several studies have been performed on their effects in-vitro and in-vivo. For the investigation of
chondroprotective action on chondrocyte gene expression, quantitative real-time RT-PCR is the method of choice. However, validation
of applied normalization strategies represents a crucial and sometimes neglected step in the analysis process. Therefore,
the present study aimed to determine the expression stability of common reference genes (ACTB, Beta actin; GAPDH, Glyceraldehyde-3-phosphate; B2M, Beta-2-microglobulin; HPRT1, Hypoxanthine phosphoribosyl-transferase I; SDHA, Succinate dehydrogenase complex, subunit A; YWHAZ, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide) under the influence of glucosamine,
curcumin and diacerein in the IL-1β-stimulated C-28/I2 chondrocyte model, using the geNorm software tool. |
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