Organogenesis in Camellia x williamsii: cytokinin requirement and susceptibility to antibiotics

Summary The genetic transformation of plants calls for efficient organogenetic methods. Both cytokinins and antibiotics were tested to evaluate shoot regeneration from internodes of in vitro plants of Camellia x williamsii cv Debbie. High regeneration rates were achieved by using thidiazuron, which turned out to be more effective than 6-benzylaminopurine. Up to 96% of explants regenerated when thidiazuron was used, whereas no more than 75% regenerated using 6-benzylaminopurine. The best average number of shoots per regenerant explant was 9.7 and 5.6 regarding respectively thidiazuron and 6-benzylaminopurine. Kanamycin, used in combination with the best performing thidiazuron concentration, completely blocked regeneration at 129 M. Cefotaxime at 524 M decreased the regeneration ability, especially when 2 day preculture was applied. The application of genetic transformation protocols as well as the main aims of genetic engineering in ornamental camellias are discussed.Abbreviations MS Murashige & Skoog - WPM Woody Plant Medium - BA 6-benzylaminopurine - TDZ thidiazuron - IBA indole butyric acid - NPTII neomycin phosphotransferase II - CaMV Cauliflower Mosaic Virus