A combined metabolic/polymerization kinetic model on the microbial production of poly(3-hydroxybutyrate) |
| |
| Authors: | Giannis Penloglou Avraam Roussos Christos Chatzidoukas Costas Kiparissides |
| |
| Affiliation: | 1. Department of Chemical Engineering, Aristotle University of Thessaloniki, P.O. Box 472, 54124 Thessaloniki, Greece;2. Chemical Process Engineering Research Institute, P.O. Box 60361, 57001 Thermi, Thessaloniki, Greece;1. Ministry of Education Key Lab of Bioinformatics, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, 100084, China;2. Center for Nano and Micro Mechanics, Tsinghua University, Beijing 100084, China;3. Beijing Key Laboratory of Protein Therapeutics, Tsinghua University, Beijing 100084, China;1. Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia;2. Manufacturing and Industrial Processes Division, Faculty of Engineering, Centre for Food and Bioproduct Processing, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia;3. Chemical Engineering, School of Engineering, Monash University, 46150 Bandar Sunway, Selangor Darul Ehsan, Malaysia;4. Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;5. Biorefinery & Bioprocess Engineering Laboratory, Department of Chemical Engineering and Material Science, Yuan Ze University, No. 135 Yuan-Tung Road, Chungli, Taoyuan, 320, Taiwan;1. Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan;2. Department of Nursing, Chang Gung University of Science and Technology, Tao-Yuan 33333, Taiwan;1. UCIBIO, REQUIMTE, Dep. de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal;2. IBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal |
| |
| Abstract: | In the present work, an integrated dynamic metabolic/polymerization kinetic model is developed for the prediction of the intracellular accumulation profile and the molecular weight distribution of poly(3-hydroxybutyrate) (P(3HB) or PHB) produced in microbial cultures. The model integrates two different length/time scales by combining a polymerization kinetic model with a metabolic one. The bridging point between the two models is the concentration of the monomer unit (i.e. 3-hydroxybutyryl-CoA) produced during the central aerobic carbon metabolism. The predictive capabilities of the proposed model are assessed by the comparison of the calculated biopolymer concentration and number average molecular weight with available experimental data obtained from batch and fed-batch cultures of Alcaligenes eutrophus and Alcaligenes latus. The accuracy of the proposed model was found to be satisfactory, setting this model a valuable tool for the design of the process operating profile for the production of different polymer grades with desired molecular properties. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
